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Slides were mounted in medium for fluorescence with DAPI for nuclear counterstaining (Vector Laboratories) and observed in Zeiss Axiovert microscope.
After several washes specimens were mounted in medium containing DAPI (Vectashield, Vector Labs, Burlingame, CA).
The immunostained cells were mounted in medium containing DAPI (Vectashield, Vector Labs, Burlingame, CA) and visualized under a Carl Zeiss LSM5 confocal microscope.
After a final rinse, coverslips were mounted in medium consisting of 90% glycerol, 10% 10X PBS and 1% DABCO and preparations sealed with nail polish.
Samples were washed, dried, and mounted in medium containing DAPI (Vector Laboratories), and imaged on an Olympus IX81 or BX51 microscope.
Tumour samples were immediately mounted in medium (Tissue Tek embedding medium, Miles Inc., Elkhart, IN 46515, USA) and immersed in liquid nitrogen.
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Prior to microscopy the coverslips were mounted in mounting medium Vectashield (Vector).
After several washes they were mounted in mounting medium containing DAPI (Vectashield, Vector Labs, Burlingame, CA).
Slides were washed in PBS twice and mounted in mounting medium.
Cells were mounted in antifade medium (0.1% p-phenylenediamine, 90% glycerol in phosphate-buffered saline).
After washes, preparations were mounted in Vectashield medium (Vector laboratories, CA, USA).
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