Sentence examples for mounted in media from inspiring English sources

Slides were then mounted in media containing 1.5 ug/mL 4′,6-diamidino-2-phenylindole (Vector Shield with DAPI Vector Laboratoriess, Burlingame, CA, USA), a fluorescent blue nonspecific nuclear stain.

Slides were mounted in media containing DAPI and images were acquired on a Leica DMI 6000 b automated inverted microscope.

To mark the position of the cell membrane in negative-image, cells were mounted in media containing red fluorescent dextran.

SH-SY5Y and S.NNMT.LP cells were incubated on poly- L-lysine-coated glass microscopy slides overnight, after which they were mounted in media under a coverslip.

Secondary detection occurred by fluorescence with the anti-rabbit Cy3 (Jackson Immunoresearch, West Grove, PA) or anti-mouse Alexa 488 (Molecular Probes, Eugene, OR) at 1 200 and mounted in media containing DAPI (Vectashield, Vector Laboratories, Burlingame, CA).

After incubation for 24 h, cells were washed several times with DPBS, fixed in 4% paraformaldehyde (PFA) for 1 h, and mounted in media containing DAPI (Vectasheild, Vector Laboratories, Burlingame, CA).

Fixative was washed 3 times with PBT and discs were mounted in mounting media (Dako Cytomation).

Coverslips were mounted in Mowiol media containing 2.5% 1,4-diazabicyclo[2.2.2]octane (DABCO)/1 µg/ml DAPI.

Upon washes in TBST, the sections were mounted in mounting media containing DAPI (Vectashield) and visualized with Zeiss Axiovert Microscope.

Sections were mounted in mounting media containing 4′,6-diamidino-2-phenylindole (DAPI) to confirm the extent of decellularization.

Sections were mounted in mounting media containing DAPI (4',6' diamino-2-phenylindole) and visualized with a fluorescence microscope (Olympus, Tokyo, Japan).