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Optical sectioning of the nylon membrane mounted in immersion oil was performed at 1 μm intervals, from 0 to 60 μm.
Optical sectioning of the ester membrane mounted in immersion oil was performed at 1 μm intervals, from 0 to 150 μm and a three-dimensional image was reconstructed from the optical serial sections.
Sections from resin-embedded tissue after HPF FS were mounted in immersion oil and imaged using a 100×/1.4 oil immersion objective.
Cells concentrated on filters were stained with DAPI (0.01%, 3 min), washed, dried, and mounted in immersion oil (Cargille FF, Cargille Laboratories, Cedar Grove, NJ, USA) under a glass coverslip.
For whole mount immunofluorescence analysis of Texas-red dextran tracer-filled vasculature, skin samples were fixed in 4% paraformaldehyde for 4 hr, dehydrated with a graded series of ethanol solutions (50 100%) and cleared with methyl salicylate, mounted in immersion oil, and viewed with a Bio-Rad MRC-1024 Confocal Microscope equipped with an Argon-Krypton Laser.
Following washes, sections were counterstained with 4',6-diamidino-2-phenylindole for 10 minutes, air-dried, mounted in immersion oil and viewed immediately.
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Brain slices were mounted in the immersion-type recording chamber under the two-photon microscope and continuously perfused with oxygenated artificial cerebrospinal fluid at 30 °C during recordings.
The microporous membranes were observed in their hydrated state, dry state or mounted in glycerol or in immersion oil.
A single needle specimen of 16 with dimensions 0.35 × 0.06 × 0.02 mm was protected by immersion in Paratone and mounted in a Cryoloop on a Rigaku-Oxford Diffraction Supernova diffractometer equipped with copper micro-source and Atlas detector.
Sections were mounted in Prolong Gold (Invitrogen) and imaged with 25× oil immersion (NA 0.8) objectives using a confocal microscope (Zeiss LSM780).
The two excitation beams were focused onto the sample plane by an oil immersion objective (Nikon Plan Fluor, 40× NA 1.30) mounted in an inverted optical microscope (Eclipse TE2000-E with a C2 Confocal Microscope scanning head, Nikon).
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