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Exact(29)
Sections were mounted in SlowFade mounting medium (InVitrogen).
Slides were mounted in SlowFade Gold antifade reagent (Invitrogen).
The stained slides were then mounted in Slowfade (Molecular Probes).
DNA was stained with DAPI and preparations were mounted in SlowFade (Molecular Probes).
Sections were washed in wash buffer and mounted in SlowFade Gold antifade reagent with DAPI (S36938; Invitrogen).
Coverslips were mounted in SlowFade with DAPI (Molecular Probes, Invitrogen, Carlsbad, CA, USA) on glass slides and analysed using Leica confocal microscopy.
Similar(31)
Dechorionation and antibody hybridization of fixed oocytes were performed as previously reported [20], but for fixed preps with only GFP, ovarioles were separated by rapidly pipetting up and down with a 1000 µl pipette, washed again in PBST, stained in 495 µl PBS plus 5 µl of 10 µg/ml DAPI for 6 minutes, then washed 5 times in PBST prior to mounting in Slowfade Gold.
After three 5-min washes in 1× PBS, cells were mounted in Vectashield (Vector) or SlowFade Gold(Invitrogen).
For immunofluorescence (IF) staining, primary and secondary antibodies were diluted in 12% BSA, and then mounted in DAPI that contained a SlowFade medium (Invitrogen).
Samples were counterstained with DAPI, mounted in anti-fade reagent in glycerol/PBS from the SlowFade Antifade Kit (Molecular Probes), and examined under a confocal microscope (Zeiss LSM510).
Six of these were mounted in casemates.
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