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After washing, slides were incubated for 2 min in 4′, 6′-diamidino-2-phenylindole (DAPI) to visualise the nuclei, washed again in PBS and mounted for visualisation by fluorescence microscopy with an Olympus IX70 (Olympus, Hamburg, Germany).
The autoradiographic emulsion was developed, sections were counterstained with toluidine blue (VWR) for Nissl substance and mounted for visualisation using bright field microscopy for the counterstain and epi-polarized illumination for the silver grains of the autoradiographic emulsion.
Similar(58)
Again, the Dako Envision system on the Dako Cytomation autostainer was used for visualisation of the antibody binding and tissue sections were counterstained using haematoxylin and mounted for light microscopy.
Over the weekend, calls mounted for restraint.
Domestically, complaints have been mounting for months.
Calls mount for Ron Calderon's resignation.
Remove the mounting for the switch.
Stained vessels were mounted prior to visualisation of endothelial surfaces en face using confocal laser-scanning microscopy (Zeiss LSM 510 META).
For visualisation of nuclei and mounting, Vectashield Mounting Medium with 4',6-diamidino-2-phenylindole (H-1200; Vector Laboratories) was used, and samples were analysed on an AxioImager fluorescence microscope (Zeiss, Jena, Germany).
Light counterstaining with Haematoxylin (Sigma) was performed on all specimens before mounting; this enabled visualisation of the tissue architecture without masking of the NiDAB staining.
After three 5-min washes in PBS, the glass coverslips were mounted onto slides using ProLong Gold antifade reagent (Life Technologies) ready for visualisation.
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