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Rings of the rabbit thoracic aorta with or without endothelium were mounted for isometric force recording.
Arterial functions were assessed with isolated vessels mounted for isometric tension recordings.
The arterial functions were assessed with endothelium-intact isolated rats vessels mounted for isometric tension recordings in organ chambers, as described previously [ 34].
Rat aortic segments (3 mm long) with and without endothelium were mounted for isometric tension recording in organ baths containing Krebs-Henseleit solution.
Coronary artery segments were mounted for isometric tension recordings in a 4-chamber wire myograph (DMT, Tissue Bath System 700MO, Aarhus, Denmark) containing 5°C PSS bubbled with 95% O2, 5% CO2.
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Over the weekend, calls mounted for restraint.
For force recording, 2-mm ring preparations were mounted in isometric myograph [ 31] (for details see Additional file 3).
Aortic rings (approximately 2 mm in length) were placed in 5 ml organ baths filled with oxygenated (95% O2 to 5% CO2) Krebs-Henseleit solution at 37°C, were mounted onto isometric force transducers (Kent Scientific, Minneapolis, MN, USA), and connected to a PowerLab data acquisition system (AD Instruments, Dunedin, New Zealand) for the detection of isometric tension, as described [ 17].
Study Design: Rings of omental artery with intact endothelium were mounted in organ chambers for isometric tension recording.
Helical strips of endothelial cell-intact strips were mounted in tissue baths for isometric tension recordings using Grass transducers and PowerLab data Acquisitions (Colorado Springs, CO, USA).
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