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Animals were counterstained with DAPI (Sigma, 3 μg/mL in PBSTx) for 1 h and mounted for imaging.
Post treatment, cells were washed three times with imaging media and mounted for imaging.
Cells were finally washed thoroughly with PBS and mounted for imaging.
The samples were rinsed with PBS and mounted for imaging with a Nikon TE2000 microscope.
In a zebrafish embryo mounted for imaging there are two main timescales of motion.
Cells were then fixed, stained with DAPI and mounted for imaging as described above.
Similar(44)
Following labeling, the larvae were washed three times in PBS prior to mounting for imaging.
For longitudinal studies, pump rates in day 6 animals were measured on growth medium and individually mounted for pharynx imaging.
Embryos were incubated at 37 °C for 1 h, washed, stained, and mounted for microscopic imaging.
Early to mid cleavage stage embryos (2-cell to 4/8-cell) and 2 and 7 dpf hatched larvae were fixed and mounted for SEM imaging.
Dehydrate and mount sections for imaging.
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