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After being washed twice in PBS, sections were mounted by use of a glycerol-based mounting medium containing DAPI Vector Laboratoriess, Peterborough, UK) to visualize DNA.
Finally, slides were washed with distilled water, counterstained with haemalum, dehydrated and mounted by use of xylene-soluble medium (DPX, Fluka, Buchs, Switzerland).
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After labeling, resuspended RBCs were allowed to attach to cover slips coated with polylysine, and the cover slips were mounted by using Aqua-Mount (Lerner Laboratories, New Haven, CT).
Sections were then counterstained in Mayer's hematoxylin (Histolab Product AB), dehydrated in graded alcohol and xylen and mounted by using Pertex (Histolab).
Whole mounts were briefly air dried, and coverslips were mounted by using Permount (Fisher Scientific, Pittsburgh, PA).
Preparations were mounted by using ProLong® Gold antifade reagent with DAPI (InVitrogen, France).
Coverslips were washed and mounted by using Vectashield mounting medium with DAPI (4′,6-diamidino-2-phenylindole; Vector Laboratories).
Slides were mounted by using Pro-Long GolDAPIth Dand, and the slides were imaged with a fluorescent microscope (Leica).
Then, the samples were washed in PBS again three times and mounted by using the Vectashield mounting medium (Vector Laboratories, Peterborough, UK).
DAPI was removed, cells washed twice with TBS, and flask bottoms were mounted by using Fluoromount-G (Southern Biotech, Birmingham, AL).
Finally, cells were washed three times with TBS for 10 min and one time with PBS and mounted by using Mowiol (Sigma-Aldrich).
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