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Coverslips were mounted as previously described [20].
Utricle and cochlea sensory epithelia were gently dissected and mounted as previously described [21], [22].
Embryos imaged by using differential interference contrast (DIC) optics were mounted as previously described [12] and imaged using a Leica DM IRB inverted microscope and an ×20 0.4 NA objective.
Hearts were removed and mounted as previously described.
For embryo experiments, four-cell-stage embryos were mounted as previously described (Bao and Murray 2011).
Primary antibody solutions and avidin B were simultaneously incubated and revealed and mounted as previously explained.
Similar(47)
In all experiments, we removed, fixed, and stained in carmine the fourth and/or fifth inguinal mammary glands as a whole mount, as previously described (Fenton et al. 2002).
Slides were then mounted as described previously [ 52].
The second mammary gland was whole mounted as described previously (Markey et al. 2001a).
Adult eyes, wings, and nota were dissected and mounted as described previously (Zhu et al. 2003).
For immunofluorescent cell staining, cells were fixed, stained and mounted as described previously [ 29].
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