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Saline wet mount and Lugols Iodine mount was done by direct microscopy and a formalin ether concentration method to detect ova, trophozoites, cysts, and larvae of intestinal parasites.
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Mounting was done with ProLong® Gold antifade reagent (Life Technologies).
Visualization, counter-staining, dehydrating and mounting was done as for the CA IX staining.
Mounting was done directly onto gel-coated slides using 10% phosphate-buffered saline/90% glycerol.
Mounting was done in Adobe Photoshop CC 2015.0.1 and when brightness and contrast was adjusted, the modifications were equally applied to all the set of images for a given marker.> -wrap-foot> Embryos were collected overnight on apple juice plates at 25ºC and then incubated for > 6 hr at 28ºC.
Surface-mount soldering was done on a standard laboratory hot plate using CHIPQUIK Sn96.5Ag3.0Cu0.5 solder paste at a temperature of 230 °C and through hole was done by hand using a Weller W60p soldering iron.
Preparation and analysis of samples for light microscopy, scanning electron microscopy, and histochemical localization of β-glucuronidase (GUS) activity in whole-mount tissue was done essentially as described [ 20, 80].
Whole mount analysis of ovules was done after fixing and clearing the inflorescence in methyl benzoate as described previously [ 38].
Whole-mount in situ hybridization was done by standard protocols [86], [87].
Mounting of colour plates was done on an Apple PowerPC with Adobe Photoshop.
DXA analysis was performed with a Hologic Discovery Wi using linear fan beam technology (analysis software version 12.7; Hologic Inc., Bedford, MA, USA); switching between two X-ray potentials (100 and 140 kVp) from an X-ray source mounted beneath the subject was done and the small animal-scanning mode was applied for all scanning.
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