Coverslip mounting, viewing and three-dimensional reconstructions were performed as described above.
We are also indebted to Drs. Bin Lu, Rosemary Broome, and the team at MedImmune Inc., Mount View, CA for their help with training on the ferret work.
cDNA libraries were constructed using a Marathon cDNA Amplification Kit (Clontech Laboratories, Mount View, CA, USA).
Cell cycle analyses were performed using a FACScan (BD Biosciences, Mount View, CA, USA).
(E ) Whole mount view revealing an absence of non-cardiac clones elsewhere in the same embryo.
Cells were washed again, resuspended in 3% paraformaldehyde and analyzed using a FACS Caliber flow cytometer (BD Bioscience, Mount View, CA, USA).
Figure 8 shows en face views of skin and rectal mucosa, and a whole mount view of the cecum showing the cecal tonsil, of control and treated chicks.
Cells were analyzed with a FACScan® flow cytometer (Becton Dickinson, Mount View, CA, USA), and 10,000 events were collected for each sample.
Analysis was performed on a Coulter Elite Flow cytometer (Becton Dickinson, Mount View, CA, USA) with Winlist and Winlist 32 software (Verity Software House, Inc., Topsham, ME, USA).
(A ) Schematic of a dorsal whole mount view of the embryonic mouse brain and (B ) Sagittal section of the mid-hindbrain region.
