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The lungs were stained with carmine solution using the standard whole mount procedure for mammary glands.
Collagen fibril arrangement and TEB structure is well preserved during the whole mount procedure and light scattering is reduced dramatically by extracting fat resulting in improved 3D structure, particularly for SHG signals originating from collagen.
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Ovarioles for whole mount procedures were dissected in PBS 0.2 M pH 6.8; fixation of the tissue and subsequent hybridization and detection reactions were carried out as previously reported [5].
However, the occlusal adjustment of a dental prosthesis is always necessary to ensure a precise intraoral fit even when the facebow transfer technique is used in the cast mounting procedure.
EEG electrodes fabricated this way will significantly reduce the duration and complexity of the mounting procedure as they eliminate the need for skin preparation and the application of conductive paste.
Only the confocal can image clearly through the depth of a sample, however it too has disadvantages, for example the tissue may shrink after dissection and can be subjected to shape distortion due to dissection and the mounting procedure.
The whole-mount procedure is gentler than histological sectioning because it does not require dehydration and paraffin or plastic embedding.
Problems with development may be aggravated by the dechorionation and mounting procedure.
The mounting procedure, rings vs. strips does not alter the behavior of the carotid media in tissue baths.
A stereomicroscope was fitted over the apparatus to view the fibre at 20 60× magnifications during the mounting procedure and during the experiment.
Considering the crowded compressive environment of the uterus in the intact animal, and considering furthermore that the same mounting procedure was followed for all specimens at all temperatures, we surmise that the observed alterations in thermal response of embryo size and shape at given temperatures are not due to the mounting procedure.
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