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Vaginal swabs were used to prepare a wet mount (detection of trichomonads and clue cells, and after KOH addition yeasts and amine smell), a Gram stain for Nugent scoring (done centrally at the Institute of Tropical Medicine in Antwerp), and to inoculate Trichomonas vaginalis cultures.
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Mounting detection cartridges on unmanned aerial vehicles that can loiter over suspected bioweapons plants has obvious advantages.
For whole-mount detection of pERK, embryos were fixed with 4% paraformaldehyde (PFA) in PBS overnight at 4°C.
Whole-mount detection of Edu+ cells showed a widespread distribution in the brain of young (5 7 weeks old) subjects 4 h after injection (Fig. 1A E).
Negative whole-mount detections were also verified after histological sections.
Embryos were then washed in 50 µl M2 media and frozen in groups of 20 for quantitative RT-PCR analysis or fixed for whole mount immunofluorescence detection of SNAI1 and SNAI2 proteins.
Cells were fixed with 4% paraformaldehyde in PBS for 60 min and then mounted for detection by confocal microscopy.
For whole-mount colorimetric detection of β-gal, BDNF lacZneo mice and BDNF lacZneo negative sibling controls were euthanized by CO2 inhalation.
On the other hand some authors found faecal preservation and subsequent staining superior to wet mount examination for detection of the trophozoite stages [ 28],[ 29].
A custom made forward detection mount with an attached photomultiplier tube (PMT) (Hamamatsu, H9305-04), and a band pass filter (transmittance = 475 - 485 nm) was employed to detect the SHG signal.
The steps for labeling of fixed cells for fluorescence microscopy are (1) sample preparation methods for using QDs, (2) mounting media, (3) detection with a fluorescence microscope, and (4) imaging QDs with a laser confocal microscope.
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