For BrdU immunofluorescence after FISH, the procedures were similar to that in whole mount animals, with slight modifications: all washes were limited to 5 min, and the BrdU signal was developed using rhodamine tyramide (Newmark and Sanchez Alvarado, 2000).
All three of the monoclonal antibodies were examined on whole mount animals and found to immunostain the nucleus and what appears to be the gonadal primordium (Figure 4C and data not shown).
Two of the monoclonal antibodies, 5G11 and 7E2, were examined on wild type whole mount animals and were found to stain in a similar pattern that had been reported for the polyclonal antibody and the GFP-constructs [70]: germline cytoplasm, intestine, pharynx, associated with the periphery of endocytic organelles (Figure 3H and data not shown; also see [71]).
All seven monoclonal antibodies immunostained whole mount animals with a pattern that had previously been reported with the polyclonal antibody [17]: it detects a dense network of puncta in the nerve ring, dorsal and ventral nerve cords (Figure 1B1 & C1 and data not shown).
All seven monoclonal antibodies immunostained whole mount animals with a pattern that had previously been reported with the polyclonal antibody and GFP-fusion constructs [59], [61], [62]: gonad germ cells, most embryonic cells, neurons, ventral nerve cord, commissures and body wall muscles (Figure 3F and data not shown).
All monoclonal antibodies, except 1C8, immunostained whole mount animals with a pattern that had previously been reported with the YFP-fusion constructs and polyclonal antibody [80], [81]: pharynx, intestine, body wall muscle, hypodermis, seam cells and motor neurons (Figure 1I and data not shown).
Unbelievable!" We joined Martha in the living room, which is decorated with mounted animals that Bela killed on various hunts.
Jewelry and airfares aren't covered, for instance, and neither are live animals or "stuffed or mounted animals, animal or fish trophies," according to Citi's Web site.
The place is full of mounted animals and real animal skulls like the vervet (a small African monkey) and the Louisiana swamp rat.
(His Oxnard office looks out on the Vintage Museum of Transportation and Wildlife, which houses his painstakingly assembled collection of antique vehicles, as well as several mounted animals).
Curators at the Grant Museum of Zoology at University College London will lead torch-lit tours of its exhibits: skeletons, mounted animals and specimens preserved in fluid will cast flickering shadows on the walls.
