Whole-mount analyses of mammary glands were realized as described elsewhere.
Mammary gland whole mount analyses revealed normal branching and ductal outgrowth in Postn nulliparous females, indistinguishable from that of control mice.
Thus, on the basis of whole-mount analyses, it is possible to assign molecular phenotypes to stem (Asingle and some Apaired; GFRα1+, PLZF+, NGN3+/− and cKIT−), progenitor (some Apaired and Aaligned; GFRα1+, PLZF+, NGN3+, cKIT+/−) and differentiating (A1 A4, intermediate, B; GFRα1−, PLZF−, NGN3+ and cKIT+) spermatogonia in rodents.
LJTY performed whole-mount analyses.
Whole-mount mammary gland outgrowth analyses of MMTVCre + Rac1 wt/wt and MMTVCre + Rac1 flx/flx animals at 9, 12 and 15 weeks confirmed that Rac1 is not required for mammary gland development during puberty (Supplementary Figure S2).
Our previous microarray and whole-mount in situ hybridization analyses of the zebrafish yquem/urod HEP model shows that six peptidase precursor genes – cpa5, ctr1l, ctrb1, ela2l, try and tryl – are significantly downregulated in this zebrafish model (Wang et al., 2007).
Consistent with the expression of the Asxl2 gene trap in the axial skeleton (Fig. 2) and with the hypothesis that the mouse Asxl2 is part of the PcG/trxG/ETP system, analyses of whole-mount skeletons of Asxl2−/− mice revealed a number of axial skeletal abnormalities that are reminiscent of skeletal phenotypes observed in PcG, trxG, ETP and Hox mutant mice (Fig. 4, summarized in Table 2).
Whole-mount in situ hybridization (WISH) analyses of the embryos (26 27 ss) uninjected or injected with dll4 MO using antisense probe for vegfr2 (also termed kdrl ) and kdr (kdrb ), a paralog of vegfr2.
Suitable single crystals for X-ray structural analyses of 1 was mounted on a glass fibre, and the respective data were collected on the diffractometer (graphite-monochromated Mo Kα radiation, λ = 0.71073 Å) at 108(2) K.
Single crystal analyses were carried out using suitable single crystals for X-ray structural analyses of 11 was mounted on a glass fiber, and the respective data were collected on oxford diffractometer (graphite-monochromated Mo Kα radiation, λ = 0.71073 Å) at 108(2) K.
