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Mice lacking IL-4 were unable to mount a normal protective response to infection, such that worm survival was increased.
However, the results we present in this report indicate that EGR-2 deficient mice mount a normal immune T cell immune response in vivo, comparable to that of WT mice.
This assumption was supported by showing that repeated administration of P140 into MRL/lpr mice transiently abolishes T cell reactivity to other regions of the U1-70K protein and to epitopes from other spliceosomal proteins [5], [6] without altering the capacity of P140-treated mice to mount a normal protective antiviral immune response [6].
Freyschmidt and colleagues reported that upon VACV infection by tail scarification, Relb knockout mice (deficient in a transcription factor belonging to the NF-κB family) have a more severe disease than WT mice and that this higher susceptibility is related to their inability to mount a normal Th1 response [11].
Contrary to our hypothesis that EGR-2 deficiency would lead to exaggerated T cells responses, we find that EGR-2 deficient mice mount a normal in vivo T cell responses with a peak and contraction face comparable to that seen in wild-type animals.
MyD88-deficient mice also mount a normal host defense response to Staphylococcus aureus infection [ 35].
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The saline-injected mice mounted a normal T-cell response and displayed no harmful side effects; the peptide-injected mice, on the other hand, had a much larger pool of active T cells.
EGR-2 deficient mice showed similar levels of this cytokine, indicating that they mounted a normal response against this parasite.
A comparable increase in cell numbers was seen in both the WT and CKO mice, indicating that EGR-2 deficient mice are capable of mounting a normal immune response against minor histocompatibility antigens.
Thus, ndg-4 is not required for mounting a normal apoptotic checkpoint response to DNA damage (Fig. S1C).
The edr2-6 plants, which carry the RPS2 resistance gene, mounted a normal hypersensitive necrosis response following infiltration with the avirulent bacterial strain carrying the AvrRpt2 gene (Fig. 4C).
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