Based on the operating characteristics of the on-board motors, a novel phase change material based motor packaging technology exploiting its huge latent heat is proposed in this paper.
This double-stranded DNA virus utilizes a unique motor to package its 19.3 kb genome into a preformed procapsid.
This review in particular illustrates, how Sf2 helicases translocate along DNA in the classic fashion of rotation; while pointing out that in the phage phi29 packaging motor, the genome packaging ATPase of thermophilic viruses (e.g. B204), and FtsK cellular nanobiomotors, the revolution mechanism is applied.
Taking advantage of these unique characteristics, Dr. Guo presented highlights from his pioneering work in 1998, which demonstrated that RNA dimer, trimer, and hexamer nanopaticles can be fabricated by re-engineering RNA molecules using the model of motor pRNA (packaging RNA), a component that gears the DNA packaging motor of bacteriophage phi29.
One of the key points to be noted is that the motor-drive package must be able to supply torques and speed which ensure that the required motion profile can be followed.
Although these four positively charged layers are nonessential for motor DNA packaging activity [ 89], they are reported to influence DNA translocation [ 89, 90].
The result suggests that a single-stranded gap in the DNA is a structural alteration that can cause the packaging motor to stop, and that the packaging direction is from 5′ to 3′ since the phi29 packaged the left end of the genome first.
The described method also responded to programmed stoichiometry changes, which were generated by assembling the phi29 DNA packaging motor from modified pRNA (DNA-packaging RNA) molecules forming a trimer of dimers or a dimer of trimers, instead of the wild-type hexamer.
This dsDNA packaging motor consists of a connector channel and packaging molecules to carry out its activities.
An integrated motor and gearbox package drives a 3.9 kN flocculator train at a water filtration plant.
