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To test for optical control of neuronal output in Drosophila, we expressed ChR2 in motor neurons using the D42-GAL4 driver [15].
To examine whether the expression of various components of the core promoter recognition complex changes upon neuronal differentiation, we induced ES cells to form motor neurons using retinoic acid (RA) and the smoothened agonist SAG as described previously (Wichterle et al., 2002).
The mitochondrial label (UAS-mtGFP) was expressed in motor neurons using the D42-Gal4 driver.
Here we report our results measuring the electrophysiological activity of SMN-deficient motor neurons using both single-cell and multi-electrode methods.
Here we report the first in vivo characterization of ß-actin function in motor neurons using a motor neuron specific ß-actin knock-out mouse.
SNARE protein synaptobrevin (Vesicle Associated Membrane Protein) tagged to GFP was expressed in motor neurons using the D42-Gal4 driver, and its axonal transport was assayed in the segmental nerves using time lapse confocal imaging in crawling third instar Drosophila larvae (Figure 9A).
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The mouse motor neurons used in said study exhibit enthusiastic interaction with the microtubes.
A previous work showed that motor neurons use Slit2 to promote their axon fasciculation by an autocrine or juxtaparacrine mechanism (Jaworski and Tessier-Lavigne, 2012).
An interesting recent study showed that in a viral model of motor neuron degeneration, axonal outgrowth and attachment to the muscle could be achieved from transplanted motor neurons by using GDNF secreting cells implanted into the sciatic nerve [56] and that insulin-like growth factor 1 (IGF1) may have specific effects on motor neuron fiber outgrowth [57].
We now further support the co-localization of sei and ppk29 by demonstrating that both genes are enriched in motor neurons by using in vivo Translating Ribosome Affinity Purification (TRAP) (new Figure 1E and new paragraph in the Results section).
(A ) Mouse embryonic stem (ES) cells were differentiated into motor neurons (MN) using embryoid body cultures (EB-MN) for 6 days and immunostained with MN markers ISL1/2 and LHX3, and neuronal marker TUJ1.
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