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Motivation: Alignment errors are still the main bottleneck for current template-based protein modeling (TM) methods, including protein threading and homology modeling, especially when the sequence identity between two proteins under consideration is low (<30%).
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Motivation: Alignment-based methods for sequence analysis have various limitations if large datasets are to be analysed.
Motivation: Alignment-free methods for sequence comparison are increasingly used for genome analysis and phylogeny reconstruction; they circumvent various difficulties of traditional alignment-based approaches.
Motivation: Alignment-based sequence similarity searches, while accurate for some type of sequences, can produce incorrect results when used on more divergent but functionally related sequences that have undergone the sequence rearrangements observed in many bacterial and viral genomes.
Motivation: Structural alignment methods are widely used to generate gold standard alignments for improving multiple sequence alignments and transferring functional annotations, as well as for assigning structural distances between proteins.
We need alignment.
Farage and Trump provided us with a master class in engagement in three ways: by appealing to their followers with passion and inspiration; presenting them with the 'what's in it for me' element which fostered motivation and alignment; and, encouraged and galvanised followers to be actively involved in their campaigns.
Motivation: Accurate alignment of large numbers of sequences is demanding and the computational burden is further increased by downstream analyses depending on these alignments.
Motivation: The global alignment of protein interaction networks is a widely studied problem.
Motivation: Multiple sequence alignments are generally reconstructed using a progressive approach that follows a guide-tree.
More familiar motivations – to ensure alignment with national health priorities; economic advantage; regional stability; reputation-building; altruism; and as a foreign policy tool – were also cited in various studies [ 13, 14].
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