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To reduce motion, the perfusate was supplemented with 10 μM blebbistatin (Sigma-Aldrich, St . Louis MO).
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A carrier liquid, the perfusate, is perfused into the probe.
The perfusion can be carried out with a single liver passage of the perfusate (non-recirculating "open" system of perfusion) or the perfusate can be recirculated ("closed" system).
Motion artifacts were abolished by adding 2,3-BDM to the perfusate (12 - 16 mmol/L final concentration).
The perfusate exiting the right atrium was clear at the completion of saline perfusion.
Slices were transferred to an upright microscope and the perfusate was maintained ~30 °C by an in-line heater.
PE (3 μM) was added to the perfusate, to induce a slow, tonic level of baseline firing (see Methods).
A bolus injection of CGRP (10 pmol) into the perfusate also caused a vasodilation.
In this study, the effect of the mass transfer of FP from the perfusate to the skin on the cumulative amount of FP passing through the skin was relatively low because of the use of suitable solutions as perfusate.
The structure of the glucuronide metabolites of capsaicin and dihydrocapsaicin appeared in the perfusate was identified by mass spectrometry.
The rate of glucose uptake was calculated as the amount of glucose removed from the perfusate normalized for heart weight and time.
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