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First, nuclear 'ratcheting', a forward and backward motion of nuclei, occurs while the nuclei migrate toward the basal side during G1-phase (after 0 min in Figure 1B).
Two pools of Bcd exist in the embryo: one in the bulk cytoplasm that is presumably affected by motion of cytoplasm before cycle 10, and one within the nuclei or energids which is presumably affected by motion of nuclei after cycle 10 in embryos exposed to the temperature step.
Frequencies (in cm-1) are given with plots and arrows to indicate direction and relative magnitude of motion of nuclei.
Two methods were used to quantify motion of nuclei in embryos between nuclear division cycles 11 13.
The dramatic motion of nuclei and its ultimate correction that we observed in embryos exposed to the temperature step raised two questions concerning the Bcd gradient.
Given the oscillatory motion of nuclei, is the Bcd gradient, which is localized to nuclei during nuclear division cycles 10 14, also oscillatory?
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Due to the natural motion of the nurse cell nucleus during oogenesis, we used a separate algorithm that calculates the motion of nucleus in space, and subtracts this from the motion of detected particles [56].
For polyatomic molecules the situation is compounded by the simultaneous motion of many nuclei.
Molecules are not rigid structures, and the motion of the nuclei within the molecular framework gives rise to vibrational energy levels.
To analyse nuclear movement during INM, we established a system that enabled us to quantitatively track the motion of individual nuclei in living tissue.
In the diabatic picture, the common energy of the two localized levels is E j (x) = ⟨ϕ j (x)| V x, q) + T̂ q |ϕ j (x)⟩ and represents the effective potential for the motion of the nuclei at xt in each of the electronic states localized near the donor and acceptor.
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