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A jet mounted on the side of the pipe disturbed the flow and a laser and cameras 6 meters downstream tracked the motion of beads in the water.
We found that the deactivation of the motor activity of myosin II structurally and functionally only affects the stress fibers, not other actin structures in the cytoplasm and does not significantly affect the motion of beads embedded in the cytoplasm.
The dynamic motion of beads on the cultured ECs was observed by time-lapse microscopy.
The motion of beads under the influence of the rotating beams is monitored from backscattered white light with a CCD camera.
More recently microrheometry using CLSM to track the motion of magnetic beads in response to an oscillating magnetic field (Galy et al., 2012) or the natural brownian motion of beads within the biofilm (Chew et al., 2014) have been used to map the viscoelastic properties of biofilms at specific locations in 3D.
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Mizuno et al. previously reported anticorrelated motions of bead pairs in reconstituted actomyosin networks, which they attributed to non-equilibrium, contractile/compressive gel deformations [3].
At the same time, the researchers shook the can up and down and used a high-speed camera to film the motion of the beads.
The motion of the beads then reorients nearby rods, which get dragged along in the flow like weathervanes in the wind, leading to the overall flocklike behavior.
Positron emission particle tracking (PEPT) was used to study the motion of glass beads in a vertical axis mixer with slowly rotating flat blades.
Indeed our results show that the spontaneous motion of the beads is largely unaffected by the bundling and contractile activities of actomyosin stress fibers, both in perinuclear and lamellar bead populations.
However, the size of individual first and non-successive strokes cannot be determined directly because the actual start positions of the steps are random due to the Brownian motion of the beads [24].
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