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After correcting for distortions due to eddy current and head motion, maps of fractional anisotropy, mean diffusivity, axial diffusivity and radial diffusivity were calculated by fitting the diffusion tensor model within each voxel using the FDT software tool in FSL.
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The videos were downsampled spatially by a factor of 2, and PCs of the video were calculated by first computing the motion map of the video as above, and then shifting an 8 frame window across this motion map.
DOI: http://dx.doi.org/10.7554/eLife.07290.005 To correlate specific movements with granule cell input and output, we performed activity-triggered averaging of motion maps in video images of locomoting mice.
Also seen in Figure 5 are the motion maps created as part of the gating+ processing.
For each of the videos, we created a data matrix where the rows contained the 8 frame windows of the motion maps.
Unlike most algorithms for motion analysis, we do not estimate magnitude of velocity vectors or obtain dense motion maps.
(D ) Spike triggered average of motion map overlaid on video of mouse.
This study was supported by the "Support Program for Long-period Ground Motion Hazard Maps of Japan" of the Ministry of Education, Culture, Sports, Science, and Technology of Japan.
The K-means clustering of motion map derives a binary segmentation separating the foreground from the background.
We cluster the motion map into k groups of regions using K-means and we set k as 10 in the experiments.
A motion index was calculated by thresholding the motion map (with a threshold of 40 at 8 bit resolution) and summing the pixels exceeding the threshold.
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