Motility was quantified as the diameter of the swimming colony.
Cell motility was quantified by measuring the distance between the migrating cell boundaries.
Antral motility was quantified by calculating the gastric motility index.
Finally, cellular motility is quantified by calculating the variances of the detected temporal shape fluctuations.
Cell motility is quantified by counting the number of cells that pass through the filter membrane.
Cell motility was quantified manually, tracking ≥35 cells/condition throughout the time stack, using the ImageJ software.
Gliding motility was quantified by determining the mean percentage ± standard deviation of parasites that exhibited gliding motility by producing characteristic CSP trails (≥1 circles) or parasites that did not produce CSP trails (0 circles).
Motility was assessed using ImageJ software (National Institutes of Health, Bethesda, MD, USA), and the average total cell motility was quantified per 50-μm Z-stack (five sections imaged at 10-μm intervals).
To confirm whether DNP promotes metastasis through LYRIC phosphorylation, 6-10B-pcDNA3.1, 6-10B-LYRIC, and 6-10B-LYRIC-mutant cells were treated with DNP, and their motility and invasiveness were quantified using the in vitro Boyden chamber invasion assay.
Filopodium extension and retraction rates were quantified using a 'Length Motility Index' (LMI; Brocco et al, 2010; Konur & Yuste, 2004).
