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Total sperm count and motility were obtained.
In general, consistent results on sperm motility were obtained.
Additionally, consistent stimulation of total and progressive motility were obtained when the cells were incubated under capacitating conditions (Fig. 3C).
Similar(57)
Gliding motility data were obtained by microcinematography as described previously [17].
> -wrap-foot> sixilarly, six mutations resulting in a lack of arsenite oxidation as compared with the wild-type and motility mutants were obtained after screening 6,000 kanamycin-resistant clones with the silver nitrate method (Muller et al. 2007) (table 2).
In terms of the formation of the lamellipodial structures after plating and the lateral cell motility, similar results were obtained using the non-tagged proteins (Additional file 1: Figure S1b d).
From the four tested temperatures (10°C, 12.5°C, 15°C and 17.5°C) during hormonal treatment, the significantly highest spermatozoa motilities (65%) were obtained at 10°C and the lowest (41%) at 17.5°C.
Movies of Cy5-scaffold motility on the actin filaments were obtained at 2 Hz for ≥30 min. Keratocytes were derived from scales of Thorichthys meeki (Firemouth Cichlids) or Rocio octofasciata (Jack Dempsey Cichlids) as previously described (Sivaramakrishnan and Spudich, 2009).
Similar motility defects, followed by premature death, were obtained after the acute silencing of the TBPH protein in adult flies (Fig. 5A D), proving the continuous necessity of TBPH function in these tissues during the Drosophila life cycle.
Higher post-thaw sperm motility duration and percentage as well as fertilization rate were obtained with the GM extender when compared to TSKM extender.
Main outcome Measure(s): Before and after treatment, standardized semen were obtained for determination of sperm concentration, motility and morphology, according to World Health Organization (WHO) guidelines.
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