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Sperm counts were expressed as the sum of five squares on the hemocytometer, and the percent motility was determined by counting both motile and immotile spermatozoa per area.
Sperm motility was determined by macerating the epididymis in mortar and pestle and mixing it with 1 ml of phosphate-buffered saline (37 °C) in order to release the sperm cells, and a drop of the suspension was quickly placed on a glass microscope slide, covered with a glass slip and examined using a light microscope at × 40 magnification.
Activity of surfactants on sperm motility was determined by the Sander Cramer assay [32].
Swimming motility was determined by measuring the diameter of the turbid zone around the inoculation site.
Rate of wound closure, as a measure of cell motility, was determined by live cell imaging using a specialized WoundMaker, ImageLock plates and Incucyte (Essen Instruments).
Cell motility was determined using two independent assays.
Similar(30)
Swimming, swarming and twitching motility were determined as described previously [ 150].
Sperm concentration, semen volume, and motility were determined at the centers, and morphology was assessed at a central laboratory.
(B ) Instantaneous speeds of microtubule motility were determined from tracks of 50 microtubules and shown as histograms.
(E ) Instantaneous speeds of microtubule motility were determined from tracks of 20 single and bundled microtubules and shown as histograms.
The choice between proliferation and motility is determined at least in part by the phosphorylation status of the NG2 cytoplasmic domain.
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