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GI motility was detected by a dye retention assay; phenol red dye was retained more in the stomachs of infected hamsters as compared to sham-infected hamsters.
However, no motility was detected.
Cell motility was detected using the Cellomics Array Scan VTI 1700 plus (Thermo Scientific) according to the manufacturer's protocol (each group included five repeated wells).
At time zero of incubation, motility was detected in 33 (46%) and 45 strains (63%) examined in H2O and TSB, respectively.
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This can be explained by the tendency to increase AI dose and thus the concentration – the volume of one straw is limited – when low post-thaw motility is detected to increase the number of progressively motile spermatozoa and consequently the possibility of pregnancy [ 35].
Turkey sperm frozen with glycerol exhibited higher membrane integrity and membrane quality upon thawing than turkey sperm frozen with DMA although no differences in total motility, and only minimal differences in progressive motility, were detected among the eight cryopreservation treatments.
No other indices of abnormal oesophageal motility were detected.
However, no significant changes in motility were detected among these cell lines (data not shown).
No rescue of the motility defect was detected in over 700 transformants from three independent experiments.
The cell motility rate was detected before freezing and after recovery.
Using the method of equation fitting, we determined the point when the first sign of sperm motility change was detected, i.e., θR and TR.
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