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Sperm motility was analyzed at 10 and 90 min after thawing, while sperm viability and acrosomal integrity were analyzed 20 min after thawing.
Spermatozoal motility was analyzed, intracellular calcium was measured, and the ability of sperm to undergo acrosome reaction was compared after incubation in a skim milk extender (SME) and Tyrode's medium containing albumin, lactate, and pyruvate (TALP) at 37 °C.
To quantify the activity at different time points post-gavage, charcoal motility was analyzed and calculated, normalizing all parameters (Figure 7A, B).
Motility was analyzed with ImageJ software and the MTrackJ plugin.
Peroxisomal motility was analyzed by animation of the entire time series using the KHOROS software package [73], [74].
In a separate set of experiments, leukocyte motility was analyzed after inhibition of Rho kinase with a selective inhibitor (Y-27632, 50 µM) [38], [30].
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The sperm directionality and motility were analyzed with the ImageJ software (ver. 1.38, NIH, USA) and the MtrackJ plugin (ver.1.1.0, Eric Meijering).
TbPFR2 expression and cell motility were analyzed.
Expression levels of 31 genes in nine operons involved in flagellar motility were analyzed.
Alterations in cell surface marker expression, cytokine secretion and chemotactic motility were analyzed using flow cytometry, enzyme-linked immunosorbent assays and chemotaxis assays, respectively.
To evaluate a potential negative effect of viral suspension on sperm quality, viability and motility were analyzed before and after mixing with viral suspension.
More suggestions(15)
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