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In brief, after background subtraction and application of an automatic threshold, the plugin automatically processes the data, detects the different motility patterns and calculates the gliding speed.
Once matched GFP+ cells were identified, they were numbered and each was examined for motility patterns and speed in the time lapse movie and for marker expression with post hoc immunohistochemistry.
Information about motility patterns and transit can also be obtained by tracking a small magnet through the gastrointestinal tract.
On the other hand, sand accumulations could potentially cause inflammation of the intestinal mucosa [ 11] with disrupted motility patterns and decreased excretion as a result.
Figures 3a-c depict representative examples of the motility patterns and kinematic parameters of fast, medium and slow moving spermatozoa among this characteristic "slow" swimming population.
MRI has also been used to track the position of fluorine labeled capsules giving information about small intestinal motility patterns and this can be combined with anatomical data [ 16].
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Clustering analysis allowed the detection of fish sperm subpopulations according to their motility pattern and showed that sperm composition in terms of subpopulations was differentially affected by the cryopreservation protocol depending on the cryoprotectant used, freezing rates and packaging systems.
Mammalian spermatozoa become fertilization-competent during capacitation in the female oviduct, a process that alters the sperm motility pattern and primes the sperm exocytotic organelle, the sperm head acrosome, for interactions with the egg zona pellucida (ZP [1], [2].
Additional file 1: Motility pattern and details of the adult Lobatocerebrum riegeri n. sp. This movie shows combined clips of alive Lobatoerebrum riegeri n. sp. indicating both morphological specificities such as the pharynx and brain and motility patterns.
Cells treated with 25 μg/ml chloramphenicol to inhibit protein synthesis displayed a motility pattern and a RomR-GFP localization pattern similar to that of untreated cells (data not shown).
Hyperactivated motility of CD1 sperm increased during capacitation reaching maximal levels of ∼20 30% by 90 min, and the percentage of nonvigorous motility patterns (slow and weakly motile) also increased during this 2-hr interval.
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