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This study was designed to test the effect of post-thaw storage time on sperm motility parameters of Caspian brown trout (n = 7).
A storage time of six hours following thawing did not have a significant effect on the motility parameters of thawed carp sperm.
Motility parameters of thawed sperm were similar using a conventional cryopreservation technique using a polystyrene box [motility (33%), VCL (47 μm/s) and STR (88%)] and a programmable freezer: [motility (32%), VCL (54 μm/s) and STR (89%)].
This agonist did not alter the gross motility parameters of sperm determined by CASA.
Even at the highest menthol concentration used (1 mM) we did not observe significant changes in the main motility parameters of non-capacitated or capacitated sperm (Supplementary Figure S1).
The use of computer-assisted sperm analysis (CASA) demonstrates that basic motility parameters of a whole sperm population can be significantly different compared to the evaluation of motility parameters on a single cell level.
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Regarding sperm treatments, PERCOLL group showed the highest values for some motility parameters (linearity of the curvilinear trajectory, straightness, and average path velocity/curvilinear velocity), ROS generation and penetration ability at 2 and 4 hpi; however this same group showed the lowest values for sperm curvilinear velocity and lateral head displacement.
On Days 1, 4 and 7 (Day 0 = day of semen collection), motility parameters and the percentage of total motile sperm and progressively motile sperm using a CASA system was evaluated.
(C ) Comparison of the motility parameters generated from the analysis of b12 L and b12 HL B cells adoptively transferred into mice previously injected with gp120.
A more detailed analysis of the various motility parameters (flagellar curvature, bending angle, frequency of beating, chemoattraction, etc).
The cells were traced and each traced sequence was analyzed using Dynamic Image Analysis Software DIASSollSoll Technologies, Inc., Iowa City, IA USA), to obtain a series of motility parameters.
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