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Boar spermatozoa require bicarbonate in the extender (to the levels present in P1) to maintain acceptable motility over a 120-h period at 16 17 °C (Exp II).
Velocity maps have been used to evaluate the kinematics of cell motility over a variety of cell types and conditions [10], [12], [14], [25] [28].
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Nexilin is a F-actin binding protein and mediates cell motility, over-expression of which promoted cell migration and adhesion[46].
Analogous to a tug-of-war, donor-treated and untreated myosins are laid down in a motility assay over a range of relative concentrations, ranging from 100% treated and 0% untreated, to 0% treated and 100% untreated.
This is consistent with the finding of motility maintenance over a significant time course, even with continuous incubation (Table I).
Previous reports have observed sporozoite motility in the dermis (Amino et al., 2006; Hellmann et al., 2011), but an in-depth analysis of their motility over time has not been performed.
To quantitatively assess sporozoite motility over the first 120 min after inoculation into the skin of a mouse, we generated P. berghei sporozoites expressing the fluorescent protein mCherry under the control of a strong sporozoite-stage promoter and visualized them in the ear pinna.
We characterize changes in sporozoite motility over the first 2 hr after intradermal inoculation and identify an altered type of sporozoite motility in proximity of blood vessels.
Centola said she combed through the past eight years of sperm-donor data in the Boston area, and found "a statistically significant decline in semen volume, sperm count and motility over those years".
Addition of hyaluronan, a nonsulfated glycosaminoglycan, to fresh and frozen thawed human semen results in substantial retention of motility over time.
This strategy leads to enhanced retention of the fibronectin fragment over adsorbed fibronectin, and increased cellular proliferation and motility over either covalently immobilized or adsorbed fibronectin.
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