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We constructed a next-generation optical trapping instrument to study the motility of single motor proteins, such as kinesin moving along a microtubule.
These proteins are crucial for cellular motility of single celled organisms and the sperm of many multicellular organisms which reproduce sexually.
Motility of single interstitially migrating leukocytes was analyzed after microinjection of MIP-1α, PAF, or E. coli as well as after intrascrotal injection of PAF.
To further characterize the effect of c-di-GMP, we measured motility of single cells in a uniform environment.
Here we show that it is possible to measure the motility of single cells in conjunction with the dry mass measurements.
To assess the role of Sec7 for motility we analyzed the motility of single cells during the aggregation phase after six hours of starvation.
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Note that processive motility of single-headed Kif1 has not been replicated in another laboratory.
To conclusively determine whether romR is required for A-motility, we analyzed movement of single cells on 1.5% agar by time-lapse microscopy.
Using the in vitro motility assay we observed the movement of single fluorescently labeled actin filaments gliding over a myosin-coated glass surface [7], [8].
Proteins that regulate epithelial polarity help orient migrating cells and promote motility of both single and collectively migrating cells by organizing the cellular membrane and cytoskeleton (Humbert et al. 2006; Etienne-Manneville 2008; Hidalgo-Carcedo et al. 2011).
Other quantification methods have been developed to study the distribution of protein and the polarity of molecular activity by dividing the whole cell into small wedges in the polar coordinated system [23], [26] [28], to study the motility and energy of single sperms by tracking and trapping [29], [30], or to extract data on 3D vascular anatomy [31].
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