Gliding motility data were obtained by microcinematography as described previously [17].
The motility data were analyzed using a one-way ANOVA test followed by a Tukey-Kramer test.
The analyses of the GETs and motility data were performed by investigators who were not aware of the test infusion conditions.
Amylovoran production and swarming motility data were undertaken a one-way ANOVA and Student-Newman-Keuls test to determine differences in means (P = 0.05) using SAS 9.1 program.
Motility data is further characterized as follows: mean curvilinear velocity (VCL), path velocity (VAP), mean straight-line velocity (VSL), straightness (STR = VSL/VAP), linearity (LIN = VSL/VCL), percentages of total motility (MOT), progressive motility (PMOT), amplitude of lateral head displacement (ALH) and beat cross frequency (BCF).
Motility data were excluded for one man whose time to semen analysis exceeded 30 min. The analysis reported here includes all 126 men with complete data on all study outcomes and covariates, including both measures of AGD, except sperm morphology data, which was available for only 124 men.
However, since ICAM-2 WT is glycosylated at this site and the WT protein significantly inhibits NB cell motility, the data are not consistent with a straightforward correlation between glycosylation of this specific residue and cell motility, as reflected by this assay.
Motility assays were performed in quadruplicate and data are expressed as mean ± SEM.
Thus, the "Worminator" provides a relatively low-cost platform for developing genera- and stage-specific assays with high efficiency and reproducibility, low labor input, and yields objective motility data that is not subject to scorer bias.
These data are consistent with our observation of an initial period of high dispersal motility, followed by more constrained motility.
