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Thus, the "Worminator" provides a relatively low-cost platform for developing genera- and stage-specific assays with high efficiency and reproducibility, low labor input, and yields objective motility data that is not subject to scorer bias.
The evaluation of the motility data obtained with a CASA system, applying a Two-Step Cluster analysis, identified in seabream sperm 3 different sperm subpopulations that correlated differently with embryo hatching rates.
Gliding motility data were obtained by microcinematography as described previously [17].
CRIB domain microinjection into cells immediately inhibited vesicle motility (data not shown).
Such exponential distributions for motility data are thought to arise from the interplay of various cellular processes combined with a finite rate of ATP production [20].
The ATPase and motility data thus show that the motor domain of NcKin performs its native activity (i.e.: processive motility) even in the presence of the non-native neck domain, and is not dominated by the neck of NcKin3.
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The system includes a wireless motility capsule, data receiver, and data analysis software GIMS Data Viewer v.1.4 and SmartPill MotiliGI, which are provided by the manufacturer.
Down-regulation of G9a expression or activity using pharmacological inhibitors reduces tumor growth and motility (unpublished data).
We also established statistical significance of the association between clusters of positively selected genes on the branch b involved in cell motility (Additional data file 5).
Since PKD1 was previously characterized as a negative regulator of cell motility, our data suggest that a PKD1 reexpression strategy may be used as a therapeutic approach to reduce or prevent breast cancer cell metastasis.
[Ca2+]i signalling is fundamental for sperm motility and data from this study will lead to assessment of the nature of these defects using techniques such as single-cell imaging and patch clamping.
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