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We next examined lateral cell motility by using a wound healing assay.
We further confirmed the effect of downregulating CPT1A on cell motility by using Etomoxir, a pharmacologic inhibitor of CPT1 [ 24].
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To determine whether PL affects the invasive ability of human colon cancer cells in vitro, we performed invasion and motility assays by using various concentrations of PL.
Based on previous reports showing that activin A and prostaglandins promote cell motility [ 22, 24- 26], we assessed the motility of vHMECs by using a cell-wounding assay.
The contribution of Tiam1 to cell motility was directly examined by using transwell motility and wound-healing assays.
We then examined the lateral motility of cells by using a wound healing assay (Fig. 1e).
Haptotactic cell motility was measured by using 8 μm cell culture inserts, as previously described.
The impact of VX-680 and ZM447439 on OS cells motility was assessed by using a wound-healing migration assay (Supplementary Figures S2A and B).
We next studied whether this protein could influence cell motility and invasion by using cell-scatter, wound-healing, and Boyden chamber assays.
The percentage of inhibition of gastrointestinal motility was determined by using the following equation: The effect of the hydroalcoholic extract on fluid secretion in intestine, which was induced by misoprostol at 20 μg/kg, was studied using mouse model.
The effect of CPT1A siRNA on cell motility was confirmed by using two different individual siRNAs, CPT1A siRNA 1 and CPT1A siRNA 2 (Additional file 4: Figure S3A and S3B).
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