Optimal time frames for harvesting testis, epididymis, ovary, and uteri without compromising the histological quality, sperm motility, and RNA quality have been determined.
Recent studies have revealed that parkin plays general role for mitochondrial motility and mitochondrial quality (Bingol et al., 2014; Gegg et al., 2010; Matsuda et al., 2010; Narendra et al., 2008; Tanaka et al., 2010) through modulating the stability of Miro (Wang et al., 2011b) and many other mitochondrial proteins (Chen and Dorn, 2013; Gegg and Schapira, 2011).
In sperm, ROS can cause potential damage to plasma membrane and DNA integrity, motility, and overall semen quality [ 2, 4, 5]; therefore, scavenging excess ROS is mandatory for normal spermatogenesis and fertilization.
Thawed sperm were evaluated for plasma membrane integrity and quality, motility, acrosome integrity and, after artificial insemination, for egg fertility and hatchability.
These videos cover a wide variety of cell types, microscopy and experimental setups, cell density and motility, resolution, image quality and dimensionality.
Chronic constipation is a common motility disorder that may affect overall wellbeing and quality of life.
Semen quality (sperm motility and morphology) from these bulls was normal.
After allowing at least 30 min for liquefaction to occur, semen analysis was performed to measure sperm concentration, sperm motility and sperm morphology using Sperm Quality Analyzer IIC (SQA IIC, United Medical Systems Inc ,Santa Ana, CA, USA) [ 29, 30].
Excessive ROS generation may adversely affect sperm number, motility, quality, and function including damage to sperm DNA integrity [ 10- 12].
We also analyzed spontaneous behavior of pups on PND 12, to evaluate overall level of activity and quality of the age-specific motility repertoire.
