We compared the effects of one concentration (100 ng/ml) of recombinant bovine GH (rbGH) and recombinant human IGF-I (rhIGF-I) on motility and motion characteristics of equine spermatozoa over a 24 h period.
A computer-assisted sperm analysis (CASA) system (sperm analysis imaging system version (SAIS -PLUS 10.1; Medical SAIS -PLUSoul, Korea) was used to analyze sperm motility and motion kinematics.
Sperm concentration, motility, and motion parameters were analyzed according to the World Health Organization (WHO 1999) guidelines using a Micro-cell slide and computer-aided semen analysis (WLJX 9000; Weili New Century Science & Tech Development Co. Ltd., Beijing, China).
Semen samples were analyzed for sperm concentration, motility, and motion parameters using a computer-aided semen analyzer (CASA; version 10HTM-IVOS; Hamilton-Thorne Research, Beverly, MA) as previously described (Meeker et al. 2004a, 2008).
To analyze motility and motion kinematics of before-capacitation sample, the sample was pre-incubated with mTCM 199 (without 10% fetal bovine serum [v/v] and 10 μg/mL heparin) for 10 min at 37°C under an atmosphere of 5% CO2 in air.
Four different tyrosine phosphorylated protein bands (approximately 18, 26, 34, and 36 kDa) were significantly increased in after-capacitation (P < 0.05, Figure 1) compare to before-capacitation spermatozoa.> -wrap-foot> Spermotilityty and motion kinematics are presented as mean ± SEM, n =3, * P <0.05.
To accurately deduce diffusion coefficients using RICS in live cells, one has to account for and subtract slow background motion (gross cellular motility) and movement of large visible vesicles or organelles that would give rise to fluctuations with an apparent diffusion of <0.002 μm/s.
Both point motion and segment motion were investigated.
(See time-and-motion study).
This compensates for translational motion from both global motion and local growth motion of the tissue.
Mostly they pointed and motioned.
