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Semen analysis was done by cell concentration, percent of motility, and quality of motility according to the World Health Organization (WHO) recommendation.
Semen samples were obtained from samples from healthy research donors (with a normal sperm concentration and motility according to WHO 2010 criteria).
Patient selection was based on diagnosis of male factor infertility induced due to low quality sperm (low concentration, morphology or motility) according to World Health Organization (WHO) guidelines [ 109].
Semen quality and the percentages of men who had below-normal values for sperm concentration, sperm count, semen volume, and percent sperm motility according to World Health Organization (WHO) criteria (WHO 1992) did not differ significantly by exposure group (see Supplemental Material, Table 2).
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Main outcome Measure(s): Before and after treatment, standardized semen were obtained for determination of sperm concentration, motility and morphology, according to World Health Organization (WHO) guidelines.
Before bringing the patient into the room, (a) perform the motility setup checklist according to policy as described in the equipment's setup protocol, and (b) make sure the patient did not eat or drink anything for 3 to 6 hours prior to test.
Volume, pH, and sperm concentration and motility were evaluated according to WHO guidelines [ 12].
The male participants will be asked to provide a semen sample which will be analysed for concentration, motility and morphology according to the WHO criteria [ 22].
The samples were analyzed for motility and concentration according to the World Health Organization (WHO 1999) manual for basic semen analysis (WHO 1999).
The samples were analysed for motility and concentration according to a manual for the project based on the WHO manual for basic semen analysis [ 24].
Samples were allowed to liquefy for 30 minutes before the evaluation of semen volume, pH, sperm concentration, viability, motility, and morphology according to World Health Organization guidelines for semen analysis [ 17].
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