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Only four transcripts had additional motifs when compared with the reference protein sequences.
The 1-kb promoters of these WRKY53-coregulated loci are enriched for the presence of W-box WRKY binding motifs when compared to the frequency distribution of all gene models across the rice genome (Fig. 3).
It is interesting to note that the Splitter peak regions are also significantly enriched for TRE motifs when compared to the promoter regions of previously reported TH-regulated genes (p < 0.003).
Similarly, the heterogeneity of gene expression and combinatorial regulation amongst the individual genes that make up these gene sets probably explains the inability to see greater enrichment of Pnt+Twi+HD-preferred motifs when compared with Pnt+Twi motifs alone (supplementary material Fig. S5C,D).
χ² And missingness were computed using PLINK v 2.050 (http://pngu.mgh.harvard.edu/purcell/plink/). 12 Array genotypes were used to identify mtDNA haplogroup-specific sequence motifs, when compared with the mtDNA reference sequence (see online supplementary materials and table S1), allowing 98.48% of subjects to be successfully assigned to a European haplogroup.
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Of particular interest in this case is H438, which can be regarded as replacement of the Sensor 2 motif, when compared to the structures of other AAA+ super family members [29].
In agreement with a more recent study, we find that DPC micelles induce helical structure and interfacial association of the SNARE motif when compared to more native environments such as bilayers and bicelles.
We therefore determined if conservation of key residues correlates with surface properties of the SPATE proteins, but there was no consistent motif when comparing hydrophobicity and/or charge of the respective linker regions of EspP, Pet and Hbp (data not shown).
An Ets-binding site is the second most overrepresented motif when comparing Jurkat versus erythroid cells and is also overrepresented in Jurkat peaks versus control sequences.
Further support for this conclusion comes from analysis of the motif composition of the newly discovered CRMs, which show substantial similarity in motif content and in some cases, motif organization, when compared with CRMs in the orthologous loci in Drosophila (again despite the absence of alignable blocks of conserved sequence).
This feature may explain their lack of motif significance when compared to the canonical core CTCF motif.
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