Exact(4)
Based on the assumption that the control motifs should have occurrence rates similar to the real motif, we selected 42 control motifs that detect similar numbers of TFBSs in the reference data set (±15%).
For each motif, we selected all the genes with at least one occurrence of the motif within their functional windows.
From this search, to refine the σ-dependent promoter motif, we selected those D. vulgaris Hildenborough promoters that are located close to the mapped RNA 5′-ends [ 15] and orthologous promoters from the other nine species.
To ask whether Msi proteins can directly bind the Jag1 mRNA and test the RBNS motif, we selected two regions of the Jag1 3′ UTR that contained the highest density of RBNS-enriched 6mers for in vitro analysis.
Similar(56)
From the output motifs, we selected three top-ranking motifs shown in Figure 5(b).
If there are multiple such motifs, we selected one that was derived in vitro, if any.
From the original 943 database motifs, we selected motifs that are highly (log2 fold enrichment >1.5 in at least one cell type) and differentially (log2 fold enrichment range >0.75) enriched in SE DHS sequences.
If the TF had only predicted motifs, we selected a motif from a highly similar TF that is: preferably derived from an in vitro method (PBM > B1H > SELEX); assigned to the cluster that contains the majority of motifs for that TF in our PWMclus analysis; consistent with known DBD preferences; and best supported by ChIP-seq or Y1H data, if available.
The first (strict) cut-off was generated using the 5% percentile of the expectation value distribution from the motifs we selected during our sequence analysis as member of the HMM., while the second (soft) cut-off was generated using the 95% percentile of the expectation value distribution from the motifs found by the HMM but belonging to the non-Rab sequences of the Ras family.
Therefore, for motif scanning, we selected motifs whose corresponding TF's expression is either uniformly high or shows positive/negative fold change.
For every TF with motif information, we selected a representative motif, or motifs if that TF appears to have multiple-binding modes (e.g., multimers), using the following scheme.
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