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We then searched for genomic regions that possessed the canonical motif sequence using MAST (Bailey & Gribskov 1998) with a P-value threshold value of <10−6.
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Significant intron polymorphisms in Exp.1 were analyzed for alterations in motif sequences, using the inbred line W64 as reference sequence (AY323283).
Bootstrap values were obtained from in-group phylogenetic analyses with D. melanogaster or A. mellifera bHLH motif sequences using NJ, MP, and ML algorithms, respectively.
Much publicly available SSR data has been derived from AG repeat motif sequences using enrichment methods involving hybridization to SSR probes [ 17, 19, 23].
Ab initio prediction of novel protein motifs from primary sequence using heuristical approaches, enumerative measures, orthologous sequences, functional annotation and statistical over-representation have all been explored using an integrative framework [ 12- 15].
In the motif enrichment analysis, those 539 motifs are identified from DNA sequence using a de novo method.
We then continue this extension to a model that allows an arbitrary number of motif occurrences in each input sequence, using a previously described cutting heuristic.
This degeneracy also makes it difficult to scan a genomic sequence using known motifs and computationally distinguish true binding sites from false positives.
A consensus PSPG motif sequence was used to search against the glycosyltransferase database generated from the collection of 235 glycosyltransferases by the BLASTX program.
To confirm the 3D-Jury results and to obtain additional functional hints by other methods, we also searched for motifs (signature sequences) using the CDD [ 25], COG [ 27] and InterPro [ 26] databases.
Analysis of the location of the 707 SSR motifs in the sequence used to design primers showed that majority of them was located in the coding region (CDS, 491) compared to 5′UTR (107) and 3′UTR (109) (Supplementary Table S1).
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