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Both motif prediction programs identified motifs corresponding to the known CREs AG, AGL3, Athb-9, O2 and RAV1 (Table 4).
To increase confidence, we utilized 2 motif prediction programs (MEME and MotifClick) to identify over-represented 8 bp motifs in the 2 kb promoter regions of NP-like genes in Populus.
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The analysis was performed using the MEME motif prediction program [ 32].
Conserved motifs among Class I FBPase, F/SBPase, and SBPase were identified by using the protein motif prediction program MEME version 4.1.0 [ 41] under maximum number of motifs - 10.
We tested all typed MYH9 SNPs between exons 2 and 41, inclusive, along with additional HapMap SNPs in this region for possible effect on splicing with the splice motif prediction program SplicePort (11, 13).
Common motifs in promoter sequences were detected using the motif prediction programme MEME [ 10].
To gain clues as to the possible function of matreshkas, all matreshka protein sequences were analysed with functional motif and signal peptide prediction programs.
Different subcellular and structure prediction programs predict different properties.
For motif prediction, we used the following three prediction programs: WeederTFBS 1.4.2 [ 60], MotifSampler 3a [ 61, 62] and PhyloCon 3.2 [ 63].
Although no conserved domains and motifs, or any homology to known structures can be found, secondary structure prediction programs repeatedly predict this sequence to be mostly composed of β-strands (apart from N-terminus signal sequence containing transmembrane helix).
Post-translational prediction programs found a motif (FKRP) weakly resembling a sumoylation target site.
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