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The analysis was performed using the MEME motif prediction program [ 32].
Conserved motifs among Class I FBPase, F/SBPase, and SBPase were identified by using the protein motif prediction program MEME version 4.1.0 [ 41] under maximum number of motifs - 10.
We tested all typed MYH9 SNPs between exons 2 and 41, inclusive, along with additional HapMap SNPs in this region for possible effect on splicing with the splice motif prediction program SplicePort (11, 13).
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Both motif prediction programs identified motifs corresponding to the known CREs AG, AGL3, Athb-9, O2 and RAV1 (Table 4).
To increase confidence, we utilized 2 motif prediction programs (MEME and MotifClick) to identify over-represented 8 bp motifs in the 2 kb promoter regions of NP-like genes in Populus.
Common motifs in promoter sequences were detected using the motif prediction programme MEME [ 10].
For motif prediction, we used the following three prediction programs: WeederTFBS 1.4.2 [ 60], MotifSampler 3a [ 61, 62] and PhyloCon 3.2 [ 63].
The motifs were identified using the ESE prediction program "ESE finder 3.0" (Cartegni et al, 2003; Smith et al, 2006).
It should be noted that 24 proteins contain a single EF-hand motif that was identified by only one prediction program and could be false positives.
Therefore, using the benchmark, statistical filters were optimized to reduce the false discovery ratio, and then remaining motifs from all programs were combined to improve motif prediction.
The N-terminus is highly hydrophobic and, according to the protein topology prediction program TMHMM [ 31], contains a transmembrane motif (P = 0.87%).
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