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The longer motif is inserted to a sequence with background distribution and the shorter motif is slid over the background/longer motif sequence.
Specifically, the 'turn' part of the helix-turn-helix motif is inserted into the active site, which maintains the WpD loop in the open state [ 28].
Because the sliding window traverses the FASTA sequence once for every sliding window, the corresponding motif is inserted or deleted in the bucket in constant time and then tested at most c possible degenerations, and as c can be considered constant, we have time complexity in order O(n).
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PsAvr3c gene without the regions encoding the signal peptide and RXLR-dEER motif was inserted into the pET32a vector (containing His tag), GmSKRPs and NbSKRP were inserted into the pGEX-4T-2 vecontainingaininGSTStagaGE Healthcare Life Scienceence).
The SV40t polyadenylation and splicing signals of the pAEC plasmid vectors were replaced by synthetic intron and synthetic rabbit β globin-based termination/polyadenylation sequences, and 5, 10, and 20 copies of the 5′-AACGTT-3′ CpG motif were inserted.
This implanted motif was inserted every 10 seconds, yielding 171 motif-repeat pairs.
Reporter mRNAs harboring the G4 of MLL4 or a mutated G4 motif was inserted in-frame with the ORF of luciferase.
When the ElxP recognition motif was inserted into the noncognate lanthipeptide precursor NisA, ElxP was able to proteolytically remove the leader peptide from NisA.
In B cells, a yeast I-SceI endonuclease motif was inserted into the genome to become a fixed locus for DSB induction; subsequently the induced DSBs were found to preferentially join to regions of active chromatin [ 10, 19].
It also forms an open, twisted, five-strand parallel β-sheet, although in AF0826, two additional β-strands forming a β-hairpin structural motif are inserted between β4 and β5 (Fig. 3A).
The LVPRGS motif was inserted between Asp180 and 181 in 28TS/180TI-caspase 28TS/180TI-caspasee structural integrity of the sequence encompassing residues 165–174 after cleaVage, in view of toe crystal structure of active caspase-3, which discloses tight interactions between this region and the other dimaintainin a hetheotetrameric arrangement [ 22].
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