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Instead of the high-scoring CATAAT, the motif is changed to ATTAAT.
The terminal CGTC motif is changed to TGTC or CCTC in many repeats.
The cysteine in the DHHC motif is changed to a serine residue in the D. melanogaster DHHC β1,4-N-acetylgalactosaminyl-transferase B pilot (GABPI).
Here, we report smFRET measurements of the conformational dynamics for T. aquaticus MutS (MutS) and a MutS mutant, in which the Glu in the Phe-Xaa-Glu mismatch recognition motif is changed to Ala (MutS-E41A), bound to different DNA mismatches.
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In one such temperature-sensitive mutant, a highly conserved asparagine residue in the sensor I motif was changed to alanine.
Lysine 767 within the ARKS motif was changed to arginine.
Three p130Cas mutants were expressed: an SD mutant (15F) in which all fifteen YxxP motif tyrosines were changed to phenylalanines, an SBD mutant (mPR) in which prolines in the SH3-binding motif are changed to alanines, and a double mutant (15F/mPR) in which both the SD and SBD are changed.
The PxDxL, PxVxS and both IxPxV amino acid motifs were changed to AxAxA, and the LxVxV motif was changed to LxAxA.
We generated a mutant in which the consensus motif was changed from VKDE to AKAA (Ago2-3mutCS).
Two C. jejuni ciaD mutants were generated; the MAP kinase docking motif was deleted (ΔMKD) in one mutant and the proline-directed phosphorylation motif was changed to an alanine (ΔS/T P) in the other mutant.
When the cysteine residues of the radical SAM motif were changed to alanines by site-directed mutagenesis, the protein was found to bind approximately eight irons and sulfides, consistent with the presence of two remaining [4Fe 4S] clusters.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com