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Based on the relationship between the motifs, 7 motif groups were designated.

These 27 strong motif groups were formed by grouping together 129 out of 179 motif-sets, and included 3208 motif occurrences.

Four sequences belonging to three different motif groups were assayed against DNA binding domains from two TFs (NAC, At3g12910 and MYB31, At1g74650) fused to the VP16 activation domain in P. patens protoplast transient expression assays.

In addition to a motif group corresponding to rice miR2118 target sites which are associated with the production of secondary siRNAs from hundreds of rice loci in the IGR [ 31], 11 motif groups were recovered from the analyses of 11 Arabidopsis and rice degradome libraries (Table  1).

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The 27 strong motif groups are labeled as 'strong motifs' in the Figures.

Seventeen motif groups are significantly enriched in the introns flanking alternate exons among the MASS genes as compared to the SASS genes (p < 1E-5, by Fisher's exact tests with Bonferroni corrections).

The motif-sets in each motif group were input to the program, which tries to build a tree showing the similarity between the motif-sets.

All strong and weak motif groups identified for each of the 13 sets of genes are given in Additional files 1, 2, 3, and 4. In order to independently check if the motif-sets grouped together to form each strong motif group were indeed similar, the web tool, STAMP [ 37], was used.

Out of these 49 sequence motifs, 15 individual cis-sequences shown in Table  1 and representing all seven motifs groups, were used for Y1H.

Each strong motif group was regarded as a potential regulatory motif.

By sorting the motif occurrences, the best subset of occurrences in each motif group was being considered.

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