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Moreover, by performing motif discovery analysis we found the highest enrichment for FOX- and SOX-family members, as well as ETS1 motifs (Fig. 4f).
For Ubx, we used the top 300 binding peaks and performed motif discovery analysis using nestedMica [50] for the embryo and haltere data separately.
In response to these questions, we present hypothetical schemes (Fig. 12) which are based on the de novo motif discovery analysis performed on the OCT4 indirect target genes postulated to be regulated under module 4 (Fig. 6 and 11A).
In order to screen for putative transcription factor binding sites other than OCT4 and SOX2, we performed a de novo motif discovery analysis based on specific promoter regions of OCT4 target genes derived from those genes which were at least 2 fold differentially regulated in NCCIT cells.
For de novo motif discovery analysis, we used the MEME or p53MH and p53Scan programs.
We performed motif discovery analysis by using clusters with differential expression.
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In other species where systematic ChIP-chip data is not available, in-silico motif discovery and analysis provide us with an alternative method.
MEME-ChIP provides several analysis tools such as motif discovery, motif enrichment analysis, motif visualization, binding affinity analysis, and motif identification.
The typical tasks include analysis of RNA-seq data (QC, preprocessing, alignment, quantitation, differential expression analysis, filtering and pathway analysis), ChIP-seq data (QC, preprocessing, alignment, peak calling, filtering, motif discovery and pathway analysis) and exome/genome-seq data (QC, preprocessing, alignment, variant calling and filtering).
The analysis of sequencing reads and TCF4 binding regions with respect to gene structure and distance to closest TSS, de novo binding motif discovery, evolutionary conservation analysis and known motif mapping was performed using Cisgenome software packages [15] and custom built Perl scripts.
All OBP and CSP sequences with intact ORF were used for motif discovery and pattern analysis.
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