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In the future, we plan on adding more accurate and efficient motif detecting programs, and optimizing the running time of the statistical methods.
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As HEAT repeat sequences are degenerated, many bona fide repeats are not detected by motif detection programs.
Motifs in the promoter regions of the differentially expressed RefSeq genes were detected using the MEME motif detection program [ 32].
If the motif width range is adjusted, is the other motif detected?
De novo motif detection using these regions by peak-motif detected ETS⇔CRE motif as the best-enriched motif.
According to the 3D protein structures, we found: 1) all the characteristic motifs locate in the crevice of GA oxidases; 2) each of the characteristic motifs consists of a loop and β sheet; 3) all the motifs detected have several positively selected or functional divergence sites by all or two programs (PAML 4.8, FunDi and GroupSim), and most of these sites are polar amino acids (Fig. 2).
Overrepresented sequence motifs identified by BEST were further used if detected by at least 2 out of the 4 motif finding programs.
The various motif discovery programs have significant limitations.
However, each of the three standalone motif discovery programs appeared to identify different sets of motifs (see Additional file 3).
The sequence motif search programs MEME (Multiple EM for motif elicitation), MDScan, and Weeder were used [ 80- 83].
Both motif prediction programs identified motifs corresponding to the known CREs AG, AGL3, Athb-9, O2 and RAV1 (Table 4).
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