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The mutations consisted in cytidine-to-thymidine transitions at a dipyrimidine motif consistent with ultraviolet (UV) light-induced damage.
Modeling of Jaburetox-2Ec, in comparison with other peptide structures, revealed a prominent β-hairpin motif consistent with an insecticidal activity based on either neurotoxicity or cell permeation.
These two motifs appear to stabilize the Rossmann fold: the first glycyl residue of either the GXXXG or GXXXA motif contacts the carbonyl oxygen atom from the first glycyl residue of the GX1 2GXXG motif consistent with the formation of a Cα H⋯O hydrogen bond.
Mouse TRF1 lacks a tankyrase binding motif, consistent with the possibility that tankyrase function in telomere maintenance differs with species (11, 16, 20, 21), and that tankyrases do not play a role in telomere length regulation in mice.
Moreover, the CTCF-binding peak is offset from the center of the M1 motif, consistent with CTCF binding to a larger, directional 33/34 bp motif.
Positions 1305 to 1313 contain a SVNGAGKST motif consistent with a Walker A loop of a MutS ATPase domain (Domain V) [ 36- 38].
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These results indicate that XB21 is a type III J-protein with motifs consistent with a role in clathrin-mediated endocytosis.
The GER4 subfamily member 5′ regulatory regions contained the most W-BOX motifs, consistent with their induction by wounding and M. oryzae.
Also, the cofactor-dependent Ubx peaks show strong enrichment of Exd and Hth motifs, consistent with a direct effect.
The GP2 nucleotide sequence contains motifs consistent with domains for a signal sequence, DC8, EGF, ZP, and one specifying a glycosylphosphatidylinositol linkage to the membrane [ 6, 1- 9].
A cartoon model showing possible scenarios for NPP motifs consistent with our observations is shown in Figure 4B and discussed below.
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