The ankyrin repeat is a protein-protein interaction motif comprised of a tandemly repeated 33 amino acid sequence.
Furthermore, Huthoff et al., employed a molecular genetic approach to map a 3 amino-acid motif, comprised of aspartic acid-proline-aspartic acid (DPD), at amino-acid positions 128 to 130 of A3G that is a crucial region for the interaction between A3G and HIV-1 Vif [31].
This structural unit defines a new motif comprised of two tryptophan side chains at the center of the hydrophobic core.
This motif (comprised of glycine-rich nonapeptide sequences) is usually located at the carboxy-terminal portion of an enzyme [ 22].
The protein sequence of NS3 is similar to NS1 protein but with an internal deletion of a motif comprised of three antiparallel β-strands spanning codons 126 to 168 in NS1.
The G motif comprised of amino acids 72 to 75 of XPA in human is well conserved across species and critical for NER as demonstrated by the lack of complementation observed using ΔG-XPA [ 48].
Recently, secondary structures of lncRNAs have been shown to be conserved, having 'repeat A' region in lncRNA Xist (X inactive-specific transcript) and a 'roX-boxes' sequence motif comprised of two lncRNAs roX1 and roX2 [ 14, 15].
The tail domain of transitin contains a motif comprised of more than 50 leucine zipper-like heptad repeats (HR domain) of the consensus sequence LQVEHGD [ 29] whereas that of nestin features an 11-amino acid repeat motif whose number of repetitions varies according to the species [ 6].
Previous chimera studies have shown that the voltage-sensor paddle, a helix-turn-helix motif comprised of S3b and S4 helices, can be transplanted between different channels that contain S1 S4 domains without disrupting voltage sensor function (Alabi et al., 2007; Bosmans et al., 2008, 2011b; Kalia and Swartz, 2013), making us optimistic that helix-5 transfer might be possible.
Additionally, a linker motif (L-motif) comprising of 7 amino acids (395KDRLKDM401) corresponds to the region that connects the C and N terminal domains of the protein in all homologs [23].
In each case, the bait protein was tagged at its carboxy terminus with the classical TAP motif, comprising elements of calmodulin binding protein and protein A separated by a TEV cleavage site [39].
